Quality Evaluation of Balinesenese Bull’s Spermatozoa in Tris Egg Yolk Extender Supplemented with Java Turmeric Extract During Pre and Post-Thawing

Using semen extender media can reduce the impact of stress on spermatozoa during cryopreservation. This research aims to analyze the effect of supplementing temulawak rhizome extract (ERT) to the Tris egg yolk extender (TKT) in Balinese bull’s semen to maintain its quality during the cryopreservation process. Four treatment groups including control/P0 (TKT + 0% ERT), P1 (TKT + 2% ERT), P2 (TKT + 4% ERT), dan P3 (TKT + 6% ERT). The research stage includes the preparation of TKT extender material, collecting Balinese bull’s semen, evaluating spermatozoa quality on fresh semen, processing frozen semen, observing spermatozoa quality evaluation, and analyzing data in the parameters of progressive motility, viability, and secondary abnormalities with one way ANOVA using SPSS ver. 17 followed by Duncan's test at 5% if a significant difference is identified. Spermatozoa viability and abnormalities analysis used smear preparations with 2% eosin-nigrosin staining, observations were carried out conventionally while motility evaluation used CASA IVOS II. Based on the research results, the addition of ERT on TKT during the freezing process has the same ability as good as the control analyzed on progressive motility and spermatozoa viability parameters. Secondary abnormalities of spermatozoa values decreased as the concentration of ERT addition to TKT increased and the lowest abnormalities was found at P3 (TKT + 6% ERT).