Study on optimization of polysaccharide deproteinization technology and antioxidant activity of ginger and its prepared products

<sec> <title>目的</title> 探究生姜及其炮制品粗多糖脱蛋白的最佳条件,比较其抗氧化能力,明确炮制方法对抗氧化能力的影响。 </sec> <sec> <title>方法</title> 利用磁力加热搅拌技术提取生姜及其炮制品中粗多糖,以磁力搅拌器转速、盐析温度和时间为因素,多糖回收率和蛋白脱除率为响应值,优化盐析法脱蛋白的最佳工艺并测定多糖含量;通过DPPH自由基清除实验等来比较不同生姜及炮制品多糖的抗氧化能力。 </sec> <sec> <title>结果</title> 最佳脱蛋白工艺条件为磁力搅拌器转速430 r/min,盐析温度50℃,盐析时间30 min,在此条件下,多糖回收率为65%,蛋白脱除率为59%。抗氧化结果表明在DPPH自由基清除实验中总多糖的抗氧化活性能力依次为炮姜&gt;生姜&gt;姜炭&gt;干姜;ABTS自由基清除实验中为姜炭&gt;生姜&gt;炮姜&gt;干姜;FRAP值测定实验中为干姜&gt;姜炭&gt;炮姜&gt;生姜。 </sec> <sec> <title>结论</title> 生姜多糖盐析法脱蛋白最佳工艺为磁力搅拌器转速430 r/min,盐析温度50 ℃,盐析时间30 min;生姜及不同炮制品总多糖含量依次为炮姜&gt;姜炭&gt;干姜&gt;生姜;综合分析炮姜的抗氧化活性最佳。 </sec>

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Publication Details

Journal
Lishizhen medicine and materia medica research
Published
2025-05-01
DOI
https://doi.org/10.70976/j.1008-0805.szgygy-2025-1011
Primary Topic
Ginger and Zingiberaceae research
Type
article
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article

Study on optimization of polysaccharide deproteinization technology and antioxidant activity of ginger and its prepared products

Zongyi Guo, Shuo Li, Jiajia Wang, Yongqi Tian et al.
Lishizhen medicine and materia medica research
Ginger and Zingiberaceae research
article

Study on optimization of polysaccharide deproteinization technology and antioxidant activity of ginger and its prepared products

Zongyi Guo, Shuo Li, Jiajia Wang, Yongqi Tian, Ping-De Niu, Xijuan Yang
article en

Abstract

<sec> <title>目的</title> 探究生姜及其炮制品粗多糖脱蛋白的最佳条件,比较其抗氧化能力,明确炮制方法对抗氧化能力的影响。 </sec> <sec> <title>方法</title> 利用磁力加热搅拌技术提取生姜及其炮制品中粗多糖,以磁力搅拌器转速、盐析温度和时间为因素,多糖回收率和蛋白脱除率为响应值,优化盐析法脱蛋白的最佳工艺并测定多糖含量;通过DPPH自由基清除实验等来比较不同生姜及炮制品多糖的抗氧化能力。 </sec> <sec> <title>结果</title> 最佳脱蛋白工艺条件为磁力搅拌器转速430 r/min,盐析温度50℃,盐析时间30 min,在此条件下,多糖回收率为65%,蛋白脱除率为59%。抗氧化结果表明在DPPH自由基清除实验中总多糖的抗氧化活性能力依次为炮姜&gt;生姜&gt;姜炭&gt;干姜;ABTS自由基清除实验中为姜炭&gt;生姜&gt;炮姜&gt;干姜;FRAP值测定实验中为干姜&gt;姜炭&gt;炮姜&gt;生姜。 </sec> <sec> <title>结论</title> 生姜多糖盐析法脱蛋白最佳工艺为磁力搅拌器转速430 r/min,盐析温度50 ℃,盐析时间30 min;生姜及不同炮制品总多糖含量依次为炮姜&gt;姜炭&gt;干姜&gt;生姜;综合分析炮姜的抗氧化活性最佳。 </sec>

Lishizhen medicine and materia medica researchVol. 36(10)
Zero hunger
Openalex Percentile: Top 74%
Ginger and Zingiberaceae research
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Study on optimization of polysaccharide deproteinization technology and antioxidant activity of ginger and its prepared products — Zongyi Guo, Shuo Li, et al. · Lishizhen medicine and materia medica research (2025) | TGRS Research Map | TGRS