In-flow digital holographic tomography for 3D phytoplankton visualization and characterization

The study and characterization of phytoplankton is of primary interest as they play a fundamental ecological role and constitute bioindicators for climate changes. Indeed, human-induced activities alter their morphology and impact their colonies. The state-of-the-art microscopy techniques allow the high-throughput visualization and enumeration of 2D images of phytoplankton and the estimation of basic parameters; however, their 3D imaging and characterization is a challenging task due to their extremely variegated morphologies that has not been fully investigated. Here, we propose for the first time in-flow digital holographic tomography as a quantitative, label free technique to recover the 3D refractive index distribution of phytoplankton, addressing the challenges related to their non-homogeneous rotations in microfluidic environment.

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Publication Details

Published
2025-08-04
DOI
https://doi.org/10.1117/12.3062743
Citations
1
Primary Topic
Digital Holography and Microscopy
Type
article
Field-Weighted Citation Impact
1.25
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article

In-flow digital holographic tomography for 3D phytoplankton visualization and characterization

Daniele Pirone, Giusy Giugliano, Lisa Miccio, Emilie Houliez et al.
1 citations
Digital Holography and Microscopy
1.25
article

In-flow digital holographic tomography for 3D phytoplankton visualization and characterization

Daniele Pirone, Giusy Giugliano, Lisa Miccio, Emilie Houliez, Jaromír Běhal, Pietro Ferraro, Valerio Zupo, Pasquale Memmolo, Maria Costantini, Vittorio Bianco, Leonilde Roselli, Francesca Borrelli, Angela Sardo
article en
1 citations

Abstract

The study and characterization of phytoplankton is of primary interest as they play a fundamental ecological role and constitute bioindicators for climate changes. Indeed, human-induced activities alter their morphology and impact their colonies. The state-of-the-art microscopy techniques allow the high-throughput visualization and enumeration of 2D images of phytoplankton and the estimation of basic parameters; however, their 3D imaging and characterization is a challenging task due to their extremely variegated morphologies that has not been fully investigated. Here, we propose for the first time in-flow digital holographic tomography as a quantitative, label free technique to recover the 3D refractive index distribution of phytoplankton, addressing the challenges related to their non-homogeneous rotations in microfluidic environment.

Stazione Zoologica Anton Dohrn (IT), Institute of Applied Science and Intelligent Systems (IT)
Openalex Percentile: Top 19%
Digital Holography and Microscopy
1.25
DOISourceOpenAlex
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In-flow digital holographic tomography for 3D phytoplankton visualization and characterization — Daniele Pirone, Giusy Giugliano, et al. · (2025) | TGRS Research Map | TGRS