MACL-1 Breast Cancer Secretome Reprograms Fibroblasts to Enhance Inflammation and Tumor Progression

Tumor-secreted factors reprogram the stromal microenvironment to facilitate cancer progression. This study investigates the effects of the MACL-1 breast cancer cell secretome on CCD19-Lu fibroblasts. Fibroblasts treated with the MACL-1 secretome exhibited increased proliferation, altered morphology, and upregulated expression of vimentin, NFκB, and fibroblast-activating protein, indicative of an inflammatory, activated phenotype. Additionally, elevated mitochondrial superoxide levels and an enrichment of proteases were detected in the secretome, but not in corresponding cell lysates. These findings suggest that the MACL-1 secretome promotes fibroblast activation and inflammation, underscoring its potential role in tumor-stroma interactions and breast cancer progression.

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Publication Details

Journal
Anais da Academia Brasileira de Ciências
Published
2025-01-01
DOI
https://doi.org/10.1590/0001-3765202520250146
Primary Topic
Cancer Cells and Metastasis
Type
article
Field-Weighted Citation Impact
0.00

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article

MACL-1 Breast Cancer Secretome Reprograms Fibroblasts to Enhance Inflammation and Tumor Progression

Jonás Perales, Andréia Barroso, Camila Dourado, Sandra Lauton‐Santos et al.
Anais da Academia Brasileira de Ciências
Cancer Cells and Metastasis
article

MACL-1 Breast Cancer Secretome Reprograms Fibroblasts to Enhance Inflammation and Tumor Progression

Jonás Perales, Andréia Barroso, Camila Dourado, Sandra Lauton‐Santos, Rodrigo Junio Rodrigues Barros, Andréia Laura Prates Rodrigues, Jáder Santos Cruz, Luciana Maria Silva
article en

Abstract

Tumor-secreted factors reprogram the stromal microenvironment to facilitate cancer progression. This study investigates the effects of the MACL-1 breast cancer cell secretome on CCD19-Lu fibroblasts. Fibroblasts treated with the MACL-1 secretome exhibited increased proliferation, altered morphology, and upregulated expression of vimentin, NFκB, and fibroblast-activating protein, indicative of an inflammatory, activated phenotype. Additionally, elevated mitochondrial superoxide levels and an enrichment of proteases were detected in the secretome, but not in corresponding cell lysates. These findings suggest that the MACL-1 secretome promotes fibroblast activation and inflammation, underscoring its potential role in tumor-stroma interactions and breast cancer progression.

Anais da Academia Brasileira de CiênciasVol. 97(3)
Universidade Federal de Minas Gerais (BR), Pontifícia Universidade Católica de Minas Gerais (BR), Universidade Federal de Sergipe (BR), Ezequiel Dias Foundation (BR), Fundação Oswaldo Cruz (BR)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Conselho Nacional de Desenvolvimento Científico e Tecnológico, Fundação de Amparo à Pesquisa do Estado de Minas Gerais
Good health and well-being
Openalex Percentile: Top 72%
Cancer Cells and Metastasis
DOIOpen Access PDFOpenAlex
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MACL-1 Breast Cancer Secretome Reprograms Fibroblasts to Enhance Inflammation and Tumor Progression — Jonás Perales, Andréia Barroso, et al. · Anais da Academia Brasileira de Ciências (2025) | TGRS Research Map | TGRS