Evaluation of the effects of clearing agents, fixation, and process durations on cardiovascular tissue imaging with second harmonic generation and multi-photon modalities
These findings establish BABB as the superior, label-free clearing agent for deep 3D multiphoton microscopy/second harmonic generation imaging of cardiovascular tissue and underscore the necessity of tailoring fixation parameters to the chosen clearing method.
Authors
- Malek Makki (ORCID: https://orcid.org/0000-0002-6574-8242)
- Gerhard A. Holzapfel (ORCID: https://orcid.org/0000-0001-8119-5775)
- Sergio A. Pineda‐Castillo (ORCID: https://orcid.org/0000-0002-9268-0597)
- Devin W. Laurence (ORCID: https://orcid.org/0000-0002-9681-4708)
- Chung‐Hao Lee (ORCID: https://orcid.org/0000-0002-8019-3329)
- Sameer Singhal (ORCID: https://orcid.org/0000-0002-3066-7573)
- Tingting Gu (ORCID: https://orcid.org/0000-0002-0696-0008)
- Zachary A. Molander
- Yasmin Eltwafsha
Institutions
- University of California, Riverside (US)
- University of Minnesota (US)
- Children's Hospital of Philadelphia (US)
- Norwegian University of Science and Technology (NO)
- Graz University of Technology (AT)
- University of Oklahoma (US)
Publication Details
- Journal
- Frontiers in Bioengineering and Biotechnology
- Published
- 2025-07-25
- DOI
- https://doi.org/10.3389/fbioe.2025.1606425
- Citations
- 1
- Primary Topic
- Advanced Fluorescence Microscopy Techniques
- Type
- article
- Field-Weighted Citation Impact
- 1.63
Funders
- American Heart Association
- National Institutes of Health
- National Heart, Lung, and Blood Institute