GATA3 Expression in Triple Negative Breast Cancer

elevated level of GATA3 in ER-positive (luminal) breast cancers.They found that GATA3 and estrogen receptor (ER) are involved in a cross-regulatory loop and are therefore regularly co-expressed in breast 3-11 cancers.But GATA3 expression in TNBC is particularly significant because this subtype of breast cancer typically tests negative for most markers associated with breast tissue.Due to low sensitivities of mammaglobin and GCDFP15, GATA3 may 12,13 potentially be a useful marker in TNBC.Therefore, this study evaluated clinical significance of GATA3 in T N B C p a t i e n t s a n d i t s c o r r e l a t i o n w i t h clinicopathological parameters and disease status. Materials and Methods Patient characteristicsThis study was approved by the Institutional Scientific Review and Ethics Committees.In this retrospective study, a total of 100 TNBC patients (ER-/PR-/HER2-) diagnosed and treated at The Gujarat Cancer and Research Institute were enrolled.The detailed clinical history of patients like age, menopausal status, histopathological findings, disease stage and treatment offered was recorded from the Institutional Medical Record Department.The disease was staged using the AJCC classification.Disease status was evaluated through clinical examination, radiological investigations and biochemical investigations. Immunohistochemical LocalizationThe tissue blocks were archived from histopathology department.Three m thin sections were taken on 3-Aminopropyl-triethoxysaline (APES) coated slides.GATA3 staining was performed on Ventana Benchmark autoimmunostainer.For IHC staining Ventana reagents were used.EZ prep solution used for deparaffinization, followed by antigen retrieval using cell conditioning 1 (CC1) buffer for 30min.Ultra view DAB detection kit was used for further IHC steps.100l GATA3 antibody Summary Background: GATA3 (GATA-binding protein 3) belongs to the zinc finger transcription factor family.It plays a role in enhancing cellular growth and differentiation across various tissues and cell types.GATA3 is an estrogen receptor (ER) regulated gene, and also present in primary and metastatic breast cancer.Aim: To evaluate clinical significance of GATA3 expression in triple negative breast cancer patients.Method: In this study, formalin fixed paraffin embedded tumour tissue from 100 patients with triple negative breast cancer were analysed in the study.GATA3 expression was assessed using immunohistochemistry method and its correlation with clinical and pathological factors, as well as disease status, was examined.Results: Nuclear GATA3 positivity was seen in 61% of triple negative breast cancer patients.GATA3 expression when correlated with clinicopathological parameters, a significant higher incidence of GATA3 expression was noted in patients with T4 tumor size, lymph node positive status and advanced stage of disease as compared to their respective counter parts.Such a correlation of GATA3 expression was not observed with other clinical and pathological parameters.In univariate survival analysis, GATA3 expression did not discriminate patients with worse or better disease free survival (DFS) and overall survival (OS).In multivariate survival analysis for disease free survival, disease stage entered at step 1 as significant prognostic factor.Conclusion: A nuclear GATA3 expression correlated with T4 tumor size, lymph node positive status, advanced disease stage suggests a marker of disease aggressiveness for TNBC.