Crystal structure of Enterovirus A71 2A protease mutant C110A containing VP1-2A junction in the active site apo structure (C1 PDB: 9FGO) used for fragment screen and compound soaking v1

This protocol describes the crystallization of Enterovirus 71 (EV-71) 2A protease mutant C110A containing the VP1-2A junction in the active site. The crystals form within 12-24 hours using a crystallization screen composed of 1.8M NaCl and 15% ethanol. The crystal structure was determined using X-ray diffraction, resulting in hexagonal prism crystals in space group P61 with unit cell dimensions of 61.5Å, 61.5Å, 78.9Å (90.00°, 90.00°, 120.00°) and an average resolution of 1.5Å. The protein was expressed using the plasmid Enterovirus Coxsackievirus A71 2A protease 228633.