Three-dimensional Culture System of Fibroblast Cells in the Fabricated Mini Bioreactor

Optimizing 3D culture is vital for developing in vitro models that mimic in vivo environments. This study evaluated static and dynamic 3D cultures of fibroblast cells encapsulated in alginate beads and examined the performance of a fabricated mini bioreactor system. Among three types of fabricated mini bioreactors – airlift, magnetic stirrer, and stirred tank mini bioreactors – the stirred tank mini bioreactor with a Rushton blade at 250 rpm and a CO2 flow of 8 mL/min showed the best conditions for cell viability (81.14%) compared to static culture (71%). These findings, supported by both trypan blue and MTT assays, highlight the benefits of dynamic agitation in enhancing nutrient distribution while preserving cell integrity. Despite promising outcomes, contamination due to inadequate sterilization of the bioreactor lid calls for design improvements. Still, this study supports the potential of optimized fabricated mini bioreactors as practical solutions for scalable and efficient 3D cell culture.