Dimensions, stability, and deformability of DOPC-cholesterol giant unilamellar vesicles formed by droplet transfer

Our findings suggest that cholesterol can lead to increased average diameter of GUVs made with DOPC through droplet transfer, while varyingly affecting their time-stability and not affecting their deformability. This study shows how small adjustments on a straightforward protocol like the droplet transfer method, provide a simple and effective way of tailoring GUV properties. Edits in the oil phase enable precise tuning of GUV membranes providing a tool for both fundamental studies and applications such as artificial cells or microrobots.