Establishment of Coala: a novel 3D and 2D cancer cell line derived from colorectal cancer liver metastasis
Metastatic colorectal cancer is one of the most critical causes of cancer-related dead in patients suffering this type of malignancy. As the liver metastases are found in almost 25-50% of all colorectal cancer patients, there is an urgent need for suitable in vitro and in vivo models. To the best of our knowledge, only a limited number of well-described 2D or 3D organoid/spheroid cell lines originated from human liver metastases was reported for this type of malignancy and submitted to ATCC and other repository centers for general use. Here, we present a novel, well-characterized cell line Coala that was derived directly from liver metastasis of patient with colorectal cancer. Coala cancer cell line was derived by 2D and 3D culture technology and can be, therefore, cultured as standard 2D cell line or as 3D spheroid culture. When transplanted into athymic mice, Coala cell line forms fast growing tumors with histological features similar to original tumor and expressed CDX2, SATB2, and CK20. Whole exome sequencing analysis of Coala cell line showed the presence of pathogenic mutations in C8B, PRKCQ, IRGM, FGFR4, POLR1C, APC and TP53 genes. As verified by antibody array chip analysis, Coala cells express Snail, Pdx-1, FoxA2, and E-cadherin. Our cell line represents a new, well characterized in vitro research tool for cancer research and will be available to researchers in the field via biorepository centers.
Authors
- Ľuboš Danišovič (ORCID: https://orcid.org/0000-0002-5074-9621)
- Sandra Meršaková (ORCID: https://orcid.org/0000-0001-5273-4434)
- Jana Melegová
- Andreas Nicodemou (ORCID: https://orcid.org/0000-0002-1989-7423)
- Mariana Brozmanová
- Dušan Braný (ORCID: https://orcid.org/0009-0009-4912-8754)
- Barbora Mitruskova
- Juraj Marcinek
- Michal Kalman (ORCID: https://orcid.org/0000-0001-8485-4254)
- Dušan Loderer (ORCID: https://orcid.org/0000-0001-5421-9831)
- Marek Mráz (ORCID: https://orcid.org/0000-0001-6975-8838)
- Andrea Horňáková
- Peter Mikolajčík (ORCID: https://orcid.org/0000-0003-0144-0668)
- Ján Janík (ORCID: https://orcid.org/0000-0002-6384-0184)
- Veronika Holubeková (ORCID: https://orcid.org/0000-0002-5979-8358)
- Ľudovít Laca
- Roman Kyčina
- Zuzana Kozovská (ORCID: https://orcid.org/0000-0002-3508-0093)
- Marian Grendár (ORCID: https://orcid.org/0000-0002-6712-3457)
- Lukáš Plank (ORCID: https://orcid.org/0000-0002-1153-1160)
- Henrieta Škovierová (ORCID: https://orcid.org/0000-0002-6854-2338)
- Erika Halášová (ORCID: https://orcid.org/0000-0003-2458-1391)
- Blažej Palkóci
- Ján Strnádel (ORCID: https://orcid.org/0000-0002-7233-6537)
- Miroslav Pindura
- Martin Vorčák (ORCID: https://orcid.org/0000-0002-1117-2356)
- Martina Poturnajova (ORCID: https://orcid.org/0000-0002-3174-290X)
- Zora Lasabová (ORCID: https://orcid.org/0000-0003-2530-3991)
- Eva Gabonova
- Miroslava Matúšková (ORCID: https://orcid.org/0000-0002-0747-9275)
- M Cermák (ORCID: https://orcid.org/0000-0001-9387-2980)
- Slavomíra Nováková (ORCID: https://orcid.org/0000-0001-6356-9665)
- Martin Vojtko
- Olga Chodelkova
Institutions
- Central European Institute of Technology (CZ)
- Institute of Chemistry of the Slovak Academy of Sciences (SK)
- Slovak Academy of Sciences (SK)
- Masaryk University (CZ)
- University Hospital Brno (CZ)
- Biomedical Research Center of the Slovak Academy of Sciences (SK)
- Msm Group (Slovakia) (SK)
- Martin University Hospital (SK)
- Comenius University Bratislava (SK)
Publication Details
- Journal
- Human Cell
- Published
- 2025-08-04
- DOI
- https://doi.org/10.1007/s13577-025-01256-1
- Primary Topic
- Cancer Cells and Metastasis
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Univerzita Komenského v Bratislave
- Agentúra na Podporu Výskumu a Vývoja
- Agentúra Ministerstva Školstva, Vedy, Výskumu a Športu SR
- European Regional Development Fund