Exploring the stability of Alzheimer's disease plasma biomarkers stored for extended periods at −20°C: Implications for resource-constrained environments
Plasma samples for Alzheimer's disease biomarker analysis are ideally stored at -80°C, which is challenging in low research resource settings. We assessed the stability of pTau181, Aβ<sub>42</sub>, Aβ<sub>40</sub>, NfL, and GFAP in plasma stored at -20°C for 2, 4, 6, and 15 weeks using single molecule array assays. No significant variation was observed in pTau181 and Aβ<sub>42</sub>/Aβ<sub>40</sub> ratio across all timepoints. NfL and GFAP showed greater variability. These findings indicate the stability of these Alzheimer's disease related analytes for up to 15 weeks at -20°C though future studies of other biomarkers and timeframes are recommended.
Authors
- Tianjie Gu
- Jamie Arvizu (ORCID: https://orcid.org/0009-0004-8733-160X)
- Farid Rajabli (ORCID: https://orcid.org/0000-0001-5229-7254)
- Patrice L. Whitehead
- Biniyam A. Ayele (ORCID: https://orcid.org/0000-0002-7955-6030)
- Jeffery M. Vance (ORCID: https://orcid.org/0000-0003-3815-8199)
- Margaret A. Pericak‐Vance (ORCID: https://orcid.org/0000-0001-7283-8804)
- C. Golightly (ORCID: https://orcid.org/0000-0001-6256-9153)
- Anthony J. Griswold (ORCID: https://orcid.org/0000-0003-1925-7810)
- Larry D. Adams
- Julianna Pascual
Institutions
- University of Miami (US)
- Dr. John T. Macdonald Foundation (US)
- Addis Ababa University (ET)
Publication Details
- Journal
- Journal of Alzheimer s Disease
- Published
- 2025-08-03
- DOI
- https://doi.org/10.1177/13872877251364899
- Citations
- 1
- Primary Topic
- Alzheimer's disease research and treatments
- Type
- article
- Field-Weighted Citation Impact
- 1.16