Analyzing size and shell stability of lipid-coated microbubbles over a fixed time span

Microbubbles are gas-filled contrast increasing agents used in ultrasound imaging, typically ranging from 1 to 10 μm in size. They are encapsulated by a protective shell to improve stability and prevent coalescence. Ensuring a stable size distribution and reliable performance over time is becoming increasingly important for their effectiveness. In this study, we explore the long-term stability and acoustic performance of our homemade microbubbles. These microbubbles are created using a mechanical agitation method, with a gas core of perfluorobutane (C4F10) and a shell made from a lipid mixture of DPPC and DPPE-PEG-2000 in a 9:1 ratio. We measure the size distribution, scattering, and attenuation of these microbubbles at regular intervals over 4 weeks. Additionally, we analyze the bubble shell properties by using the size and attenuation data. This research provides insights into the lifespan and stability of polydisperse microbubbles over expanded periods, highlighting their potential as efficient ultrasound contrast agents.