Exploring phytoconstituents of Chloroform and Ethanolic extract of F. indica and their antibacterial potency

Natural plant products have found a place in traditional systems of healing across the globe since ages. Recently, they have only been applied as isolated and described molecules for modern drug discovery and development. The current study aimed at studying the antibacterial properties as well as phytochemicals of Flacourtia indica. Alkaloids, flavonoids, glycosides, phenols, steroids, tannins, and terpenoids were found to be the main phytoactive compounds, on phytochemical screening. For the purpose of quantifying Gallic acid and Quercetin in the chloroform and ethanolic extract of F. indica, a modest, trustworthy, and predictable high-performance thin-layer chromatography (HPTLC) technique was developed. Densitometry analysis was executed out in absorption mode at 254 and 366 nm, with a scanning speed of 100 mm/s and a 6 × 0.45 nm micro-slit dimension. Presence of Alcohol phenols, Aliphatic amines Alkenes, Aromatics, Nitro compounds, Alcohol, Alkyl halides, Carboxylic acid, Esters, Ethers, Primary, Secondary amines were confirmed through FTIR analysis, with peaks at 3326.85 cm−1, 2974.87cm−1, 2853.37cm−1, 1462.49cm−1. Antibacterial activity of the extract was observed against both Gram positive and Gram negative bacteria such as Staphylococcus aureus, Streptococcus pneumoniae, Corynaebacterium kroppenstedtii, Pseudomonas aeruginosa, Vibrio cholerae and Escherichia coli by Agar Cup Well method. Minimum Inhibitory Concentration of the extract was observed in Serial dilution method using 96 well microtiter plate using Resazurin dye. Potent antibacterial activity against both Gram positive and Gram negative bacteria was shown by F. indica which creates opportunities to go for pharmaceutical research activities on the plant which has been used as traditional medicine since long time.