Comparative analysis of next-generation sequencing and immunohistochemistry in msi/mmr testing
A strong correlation exists between IHC-based MMR loss and NGS-based MSI detection. IHC remains widely used due to its accessibility and cost-effectiveness, whereas NGS offers higher accuracy and broader genomic insights. With its ability to detect multiple alterations simultaneously, NGS is particularly valuable when tissue is scarce. Combining both methods can improve diagnostic accuracy and guide optimal immunotherapy selection.
Authors
- Ayşe Armutlu (ORCID: https://orcid.org/0000-0001-9804-0454)
- Orhun Çığ Taşkın (ORCID: https://orcid.org/0000-0002-6668-3006)
- İbrahim Kulaç (ORCID: https://orcid.org/0000-0003-2003-7567)
- Makbule Aydın (ORCID: https://orcid.org/0000-0002-4541-793X)
- Burcu Saka (ORCID: https://orcid.org/0000-0002-2043-2041)
- Zeynep Seçil Satilmis (ORCID: https://orcid.org/0009-0003-8005-5553)
- Fatma Esrefi
- Gulsum Caylak (ORCID: https://orcid.org/0009-0000-9995-6608)
Institutions
- Koç University (TR)
Publication Details
- Journal
- Turkish Journal of Pathology
- Published
- 2025-01-01
- DOI
- https://doi.org/10.5146/tjpath.2025.14079
- Primary Topic
- Cancer Genomics and Diagnostics
- Type
- article
- Field-Weighted Citation Impact
- 0.00