Evaluation of Different Extenders on the Longevity and Quality of Muscovy Duck (Cairina moschata) Semen During Storage

This study aimed to evaluate the effectiveness of four different extenders-Beltsville Poultry Semen Extender (BPSE), Phosphate-Buffered Saline (PBS), Ringer's Lactate (RL), and 0.9% Sodium Chloride (NaCl)-on the longevity and quality of Muscovy duck (Cairina moschata) semen stored at 5C.Semen was collected from four mature, fertile drakes and evaluated for macroscopic and microscopic quality prior to treatment.Pooled semen was diluted to a concentration of 20010 spermatozoa/mL in each extender and stored for 72 hours.Semen quality was assessed at 0, 12, 24, 36, 48, 60, and 72 hours based on motility, viability, abnormality, and plasma membrane integrity (PMI).The results revealed that RL and BPSE were significantly more effective than PBS and NaCl in preserving sperm quality across all parameters.RL achieved the highest sperm longevity (50.4 hours), followed by PBS and NaCl (45.6 hours), while BPSE showed the shortest longevity (33.6 hours).RL also preserved the highest motility and viability and exhibited the lowest abnormality rate and best PMI over time.The findings suggest that Ringer's Lactate and BPSE are promising extenders for short-term storage of Muscovy duck semen and may serve as effective bases for further extender optimization in avian artificial insemination programs.