Fiber-Based OCT Guided Subretinal Injection in Ex-Vivo Mice Eyes
We present the use of a fiber-based Optical Coherence Tomography (OCT) system for precise targeting of the subretinal space in subretinal injection (SI). The system integrates OCT probes with a 33G needle, enabling real-time visualization of the retinal layers during SI in ex-vivo mice eyes. The setup includes a micromanipulator, an injection pump, and a software interface for user-friendly operation. This OCT-controlled system aims to standardize SI in mice, facilitating accurate preclinical validation of retinal gene therapies.
Authors
- Christos Boutopoulos (ORCID: https://orcid.org/0000-0002-3892-8483)
- Bruno Larrivée (ORCID: https://orcid.org/0000-0003-1388-3108)
- Saeid Davani
Institutions
- Hôpital Maisonneuve-Rosemont (CA)
Publication Details
- Published
- 2025-05-20
- DOI
- https://doi.org/10.1109/pn66844.2025.11097167
- Primary Topic
- Optical Coherence Tomography Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Canadian Institutes of Health Research