Comparative Analysis of Polarization Techniques in Second Harmonic Generation Microscopy

This study evaluates three polarization-resolved second harmonic generation (PSHG) techniques for collagen characterization in tendon fibrils and tissue, including Polarization-In (PI), Polarization-In Polarization-Out (PIPO), and Circular-In Linear-Out (CILO). CILO enables much faster data acquisition, and it exhibits greater variability while PIPO has slower data acquisition and demonstrates lowest variability.