A Method for the High Salt Treatment of Dendritic Cells and their Adoptive Transfer into Mice

Source: Van Beusecum, J. P., et al. Isolation and Adoptive Transfer of High Salt Treated Antigen-presenting Dendritic Cells. J. Vis. Exp. (2019). This video demonstrates a procedure for the adoptive transfer of high salt-treated antigen-presenting dendritic cells (DCs) into mice. Murine CD11c+ dendritic cells (DCs) are exposed to a medium containing high amounts of sodium, forming isolevuglandin (IsoLG)-protein adducts in the cells. These adducts serve as neoantigens and are presented on the surface of DCs. The high salt-treated cells are injected into mice through adoptive cell transfer.