Intradermal Immunization with Live Attenuated Sporozoites in a Mouse Model

Source: Mac-Daniel, L., et al. Myeloid Cell Isolation from Mouse Skin and Draining Lymph Node Following Intradermal Immunization with Live Attenuated Plasmodium Sporozoites. J. Vis. Exp. (2016). This video demonstrates an assay for immunizing a mouse model with live attenuated sporozoites. The radiation-attenuated Plasmodium sporozoites are injected into the dorsal side of the mouse's ear. After injections at multiple locations on both ears, the mouse is allowed to recover.

Publication Details

Journal
Journal of Visualized Experiments
Published
2025-07-24
DOI
https://doi.org/10.3791/30984
Primary Topic
Transgenic Plants and Applications
Type
article
Field-Weighted Citation Impact
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article

Intradermal Immunization with Live Attenuated Sporozoites in a Mouse Model

Journal of Visualized Experiments
Transgenic Plants and Applications
article

Intradermal Immunization with Live Attenuated Sporozoites in a Mouse Model

article en

Abstract

Source: Mac-Daniel, L., et al. Myeloid Cell Isolation from Mouse Skin and Draining Lymph Node Following Intradermal Immunization with Live Attenuated Plasmodium Sporozoites. J. Vis. Exp. (2016). This video demonstrates an assay for immunizing a mouse model with live attenuated sporozoites. The radiation-attenuated Plasmodium sporozoites are injected into the dorsal side of the mouse's ear. After injections at multiple locations on both ears, the mouse is allowed to recover.

Journal of Visualized Experiments
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Openalex Percentile: Top 79%
Transgenic Plants and Applications
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Intradermal Immunization with Live Attenuated Sporozoites in a Mouse Model · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS