An Avidin-Biotin Conjugation Technique for Presenting Target Antigens on <em>Mycobacterium bovis</em> BCG

Source: Liao, T. A., et al., Expression of Exogenous Antigens in the Mycobacterium bovis BCG Vaccine via Non-genetic Surface Decoration with the Avidin-biotin System. J. Vis. Exp. (2018). The video demonstrates a technique for loading antigens on Mycobacterium bovis BCG to improve its immunogenic properties. The method uses the avidin-biotin system to coat the bacterial surface with exogenous antigens.

Publication Details

Journal
Journal of Visualized Experiments
Published
2025-07-24
DOI
https://doi.org/10.3791/30978
Primary Topic
Tuberculosis Research and Epidemiology
Type
article
Field-Weighted Citation Impact
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article

An Avidin-Biotin Conjugation Technique for Presenting Target Antigens on Mycobacterium bovis BCG

Journal of Visualized Experiments
Tuberculosis Research and Epidemiology
article

An Avidin-Biotin Conjugation Technique for Presenting Target Antigens on Mycobacterium bovis BCG

article en

Abstract

Source: Liao, T. A., et al., Expression of Exogenous Antigens in the Mycobacterium bovis BCG Vaccine via Non-genetic Surface Decoration with the Avidin-biotin System. J. Vis. Exp. (2018). The video demonstrates a technique for loading antigens on Mycobacterium bovis BCG to improve its immunogenic properties. The method uses the avidin-biotin system to coat the bacterial surface with exogenous antigens.

Journal of Visualized Experiments
Zero hunger
Openalex Percentile: Top 77%
Tuberculosis Research and Epidemiology
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An Avidin-Biotin Conjugation Technique for Presenting Target Antigens on <em>Mycobacterium bovis</em> BCG · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS