Multiplexed Quantitative Bacterial Detection using Dye-Labeled Magnetic Microspheres

This video demonstrates multiplexed quantitative bacterial detection employing carboxylated magnetic polystyrene microspheres dyed into distinct sets. The procedure encompasses the formation of bead-bacteria complexes, magnetic separation, and flow-based analysis, enabling precise and simultaneous quantification of multiple bacteria in the same reaction well.

Publication Details

Journal
Journal of Visualized Experiments
Published
2025-07-24
DOI
https://doi.org/10.3791/30916
Primary Topic
Biosensors and Analytical Detection
Type
article
Field-Weighted Citation Impact
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article

Multiplexed Quantitative Bacterial Detection using Dye-Labeled Magnetic Microspheres

Journal of Visualized Experiments
Biosensors and Analytical Detection
article

Multiplexed Quantitative Bacterial Detection using Dye-Labeled Magnetic Microspheres

article en

Abstract

This video demonstrates multiplexed quantitative bacterial detection employing carboxylated magnetic polystyrene microspheres dyed into distinct sets. The procedure encompasses the formation of bead-bacteria complexes, magnetic separation, and flow-based analysis, enabling precise and simultaneous quantification of multiple bacteria in the same reaction well.

Journal of Visualized Experiments
Openalex Percentile: Top 82%
Biosensors and Analytical Detection
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Multiplexed Quantitative Bacterial Detection using Dye-Labeled Magnetic Microspheres · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS