Generating an Ex Vivo Ovine Wounded Skin Model Infected with a Bacterial Pathogen

Source: Regan, H. C., et al. A Novel High-Throughput Ex Vivo Ovine Skin Wound Model for Testing Emerging Antibiotics. J. Vis. Exp. (2022). The video demonstrates the generation of an ex vivo ovine skin model infected with Staphylococcus aureus. This entails treating a lamb's forehead skin biopsy section with antibiotics, establishing a wound bed, positioning it on a permeable membrane in a multiwell plate with antibiotic-free medium, and establishing bacterial infection in the skin wound.