Immunostaining of Neurons in Mouse Brain Slices Following Fluorescence In Situ Hybridization

Source: Baleriola, J., et. al., Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization. J. Vis. Exp. (2015)This video demonstrates the detection of mRNA within cholinergic axons in fixed mouse hippocampal slices using fluorescent in situ hybridization and antibody staining. Fluorescence microscopy reveals the spatial localization of probe-tagged mRNAs and choline acetyltransferase in cholinergic neurons.

Publication Details

Journal: Journal of Visualized Experiments
Published: 2025-07-24
DOI: https://doi.org/10.3791/31488
Primary Topic: bioluminescence and chemiluminescence research
Type: article
Field-Weighted Citation Impact: 0.00

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article

Immunostaining of Neurons in Mouse Brain Slices Following Fluorescence In Situ Hybridization

Jul 24, 2025

Journal of Visualized Experiments

bioluminescence and chemiluminescence research

article

Immunostaining of Neurons in Mouse Brain Slices Following Fluorescence In Situ Hybridization

July 24, 2025article en

Abstract

Journal of Visualized Experiments

Zero hunger

Openalex Percentile: Top 83%

bioluminescence and chemiluminescence research

DOI Source OpenAlex

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Immunostaining of Neurons in Mouse Brain Slices Following Fluorescence In Situ Hybridization · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS