Modeling Neonatal Intraventricular Hemorrhage Through Intraventricular Injection of Hemoglobin

Source: Miller, B. A., et al. Modeling Neonatal Intraventricular Hemorrhage Through Intraventricular Injection of Hemoglobin. J. Vis. Exp. (2022). This video demonstrates a method to generate a rat model of neonatal intraventricular hemorrhage. In this procedure, an anesthetized rat pup is injected with hemoglobin into the lateral ventricle of the brain. The hemoglobin causes oxidative stress and releases heme, simulating intraventricular hemorrhage. The resulting damage to brain tissues, driven by reactive oxygen species and inflammatory cytokines, leads to ventricular enlargement, a common consequence of intraventricular hemorrhage.