Fluorescence Staining of Neural Crest Cells Cultured on Hydrogels of Varying Stiffness

Source:  Le, T. P., et al., An Optimized O9-1/Hydrogel System for Studying Mechanical Signals in Neural Crest Cells. J. Vis. Exp. (2021) In this video, we describe the procedure to perform fluorescence staining using fluorescence microscopy to demonstrate phalloidin staining of neural crest cells cultured on hydrogels of varying stiffness to visualize and analyze cytoskeletal organization.

Publication Details

Journal: Journal of Visualized Experiments
Published: 2025-07-24
DOI: https://doi.org/10.3791/31430
Primary Topic: Tissue Engineering and Regenerative Medicine
Type: article
Field-Weighted Citation Impact: 0.00

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article

Fluorescence Staining of Neural Crest Cells Cultured on Hydrogels of Varying Stiffness

Jul 24, 2025

Journal of Visualized Experiments

Tissue Engineering and Regenerative Medicine

article

Fluorescence Staining of Neural Crest Cells Cultured on Hydrogels of Varying Stiffness

July 24, 2025article en

Abstract

Journal of Visualized Experiments

Openalex Percentile: Top 69%

Tissue Engineering and Regenerative Medicine

DOI Source OpenAlex

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Fluorescence Staining of Neural Crest Cells Cultured on Hydrogels of Varying Stiffness · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS