Obtaining a Single-Cell Suspension from Frozen Rat Brain Tissue

Source: Rubio, F. J., et. al. Fluorescence Activated Cell Sorting (FACS) and Gene Expression Analysis of Fos-expressing Neurons from Fresh and Frozen Rat Brain Tissue. J. Vis. Exp. (2016) This video demonstrates the dissociation of frozen rat brain tissue into a single-cell suspension using enzymatic digestion and mechanical trituration. The prepared cells are fixed and permeabilized with ethanol for downstream applications like flow cytometry or gene expression analysis.

Publication Details

Journal: Journal of Visualized Experiments
Published: 2025-07-24
DOI: https://doi.org/10.3791/31439
Primary Topic: 3D Printing in Biomedical Research
Type: article
Field-Weighted Citation Impact: 0.00

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article

Obtaining a Single-Cell Suspension from Frozen Rat Brain Tissue

Jul 24, 2025

Journal of Visualized Experiments

3D Printing in Biomedical Research

article

Obtaining a Single-Cell Suspension from Frozen Rat Brain Tissue

July 24, 2025article en

Abstract

Journal of Visualized Experiments

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3D Printing in Biomedical Research

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Obtaining a Single-Cell Suspension from Frozen Rat Brain Tissue · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS