In Vitro Assessment of Aggregated Amyloid-β on Neuronal Growth Cone Collapse

Source: Kuboyama, T. Visualizing Axonal Growth Cone Collapse and Early Amyloid β Effects in Cultured Mouse Neurons. J. Vis. Exp. (2018) This video demonstrates an in vitro assessment of neuronal growth cone collapse by amyloid-β (Aβ) aggregates. Treating the neurons with Aβ aggregates causes cytoskeletal destabilization, leading to the collapse of growth cones at the tip of the axons. The treated cells are compared with control cells to assess the extent of growth cone collapse.