Inducing Orofacial Neuropathic Pain in a Rat Model via Infraorbital Nerve Ligations

Source: Donertas-Ayaz, B. et al., Assessment of Nerve Injury-Induced Mechanical Hypersensitivity in Rats Using an Orofacial Operant Pain Assay. J. Vis. Exp. (2022) This video demonstrates the induction of orofacial neuropathic pain in a rat model by ligating the infraorbital nerve. This results in nerve damage and inflammation, causing abnormal pain signaling and mimicking chronic pain conditions, which can be used to study the orofacial pain assay.