Electrophysiological Recordings of Brainstem-Spinal Cord Preparations from a Newborn Rodent

Source: Rousseau, J., et al. Electrophysiology on Isolated Brainstem-spinal Cord Preparations from Newborn Rodents Allows Neural Respiratory Network Output Recording. J. Vis. Exp. (2015). This video demonstrates electrophysiological recordings of isolated brainstem-spinal cord preparations from newborn rodents. The preparation is placed in a recording chamber, and the inspiratory bursts from the brainstem are recorded under oxygenated and oxygen-deprived conditions using the fourth ventral nerve root in the spinal cord.