Visualization of Amyloid-Beta Peptides in Human Cortical Brain Slices

Source: Ikegawa, M. et al. Visualization of Amyloid β Deposits in the Human Brain with Matrix-assisted Laser Desorption/Ionization Imaging Mass Spectrometry. J. Vis. Exp. (2019) The video demonstrates a procedure for preparing human cortical brain slices from Alzheimer's disease patients for analyzing the deposition of amyloid-beta (Aβ) peptides using Matrix-assisted Laser Desorption/Ionization Imaging Mass Spectrometry (MALDI-IMS). The steps include preparing the slides, treating the tissue to enhance ionization, and analyzing the spatial distribution of amyloid-beta peptides.