Obtaining Cell Suspensions from Ischemic Mouse Brain Tissues

Source: Pösel, C., et al. Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain. J. Vis. Exp. (2016) This video demonstrates the procedure for isolating brain cells and infiltrated immune cells from ischemic mouse brain tissues by enzymatic digestion, followed by the removal of cell debris and myelin by density gradient centrifugation, to study inflammation responses in stroke research.

Publication Details

Journal: Journal of Visualized Experiments
Published: 2025-07-24
DOI: https://doi.org/10.3791/31380
Primary Topic: Molecular Biology Techniques and Applications
Type: article
Field-Weighted Citation Impact: 0.00

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article

Obtaining Cell Suspensions from Ischemic Mouse Brain Tissues

Jul 24, 2025

Journal of Visualized Experiments

Molecular Biology Techniques and Applications

article

Obtaining Cell Suspensions from Ischemic Mouse Brain Tissues

July 24, 2025article en

Abstract

Journal of Visualized Experiments

Openalex Percentile: Top 83%

Molecular Biology Techniques and Applications

DOI Source OpenAlex

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Obtaining Cell Suspensions from Ischemic Mouse Brain Tissues · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS