Imaging and Quantification of Protein Aggregates in Genetically Modified <em>Drosophila </em> Brains

Source: Donnelly, K. M., et al. Monitoring Cell-to-cell Transmission of Prion-like Protein Aggregates in Drosophila Melanogaster. J. Vis. Exp. (2018) This video demonstrates imaging and quantification of protein aggregates in genetically modified Drosophila brains. Brains are fixed, washed, and treated with an antifading agent before mounting. Images are captured using a confocal microscope and quantified to observe the transmission of mutant protein aggregates.