Measuring Neural Activity in a Mouse Brain Slice after Prolonged Incubation

Source: Cameron, M. A., et al., Prolonged Incubation of Acute Neuronal Tissue for Electrophysiology and Calcium-imaging. J. Vis. Exp. (120), (2017) This video demonstrates a technique for measuring neuronal activity in mouse brain slices using calcium imaging and electrophysiological recordings. By perfusing the tissue with potassium ions and recording intracellular calcium changes through high-speed digital imaging, this method reveals real-time neuronal responses.