Investigating a Zebrafish Larval Brain Injury Using Large-Scale Scanning Transmission Electron Microscopy

Source: Kuipers, J. et al., Large-scale Scanning Transmission Electron Microscopy (Nanotomy) of Healthy and Injured Zebrafish Brain. J. Vis. Exp. (2016). This video demonstrates a method to investigate zebrafish larval brain injury using large-scale scanning transmission electron microscopy (STEM), a technique that combines elements of both scanning electron microscopy (SEM) and transmission electron microscopy (TEM) to produce high-resolution images. It outlines the steps involved in preparing samples and the acquisition of high-resolution STEM images to identify brain injury.