Infecting Primary Mouse Midbrain Neurons with Engineered Adeno-Associated Viruses

Source: Bancroft, E. A., et al., Quantifying Spontaneous Ca2+ Fluxes and their Downstream Effects in Primary Mouse Midbrain Neurons. J. Vis. Exp. (2020). This video shows infecting primary mouse midbrain neurons with Adeno-Associated Viruses carrying a fluorescent calcium indicator. The indicator's fluorescence confirms successful infection and enables real-time monitoring of neuronal infection.