Electrophysiological Recordings of Reprogrammed Neurons in a Mouse Brain Slice

Source: Pereira, M., et al. In Vivo Direct Reprogramming of Resident Glial Cells into Interneurons by Intracerebral Injection of Viral Vectors. J. Vis. Exp. (2019) This video demonstrates a protocol to evaluate the maturation and reprogramming of glial cells into neurons in a transfected mouse striatum slice. The experiment measures resting membrane potential and response to current injections using neuron-specific fluorescent markers and electrophysiological techniques. Voltage spikes during these measurements indicate successful neuronal maturation and reprogramming.