Determining a Test Growth Hormone Effect on Kisspeptin Neurons using a Whole-Cell Patch Clamp

Source: Silva, J. d. N., et al., Hypothalamic Kisspeptin Neurons as a Target for Whole-Cell Patch-Clamp Recordings. J. Vis. Exp. (2023) This video demonstrates the use of the whole-cell patch-clamp technique to measure responses in kisspeptin neurons from transfected mouse hypothalamic slices. The process involves achieving whole-cell configuration and recording membrane potential changes induced by the test hormone using current-clamp mode.

Publication Details

Journal
Journal of Visualized Experiments
Published
2025-07-24
DOI
https://doi.org/10.3791/31210
Primary Topic
Plant Reproductive Biology
Type
article
Field-Weighted Citation Impact
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article

Determining a Test Growth Hormone Effect on Kisspeptin Neurons using a Whole-Cell Patch Clamp

Journal of Visualized Experiments
Plant Reproductive Biology
article

Determining a Test Growth Hormone Effect on Kisspeptin Neurons using a Whole-Cell Patch Clamp

article en

Abstract

Source: Silva, J. d. N., et al., Hypothalamic Kisspeptin Neurons as a Target for Whole-Cell Patch-Clamp Recordings. J. Vis. Exp. (2023) This video demonstrates the use of the whole-cell patch-clamp technique to measure responses in kisspeptin neurons from transfected mouse hypothalamic slices. The process involves achieving whole-cell configuration and recording membrane potential changes induced by the test hormone using current-clamp mode.

Journal of Visualized Experiments
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Openalex Percentile: Top 83%
Plant Reproductive Biology
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Determining a Test Growth Hormone Effect on Kisspeptin Neurons using a Whole-Cell Patch Clamp · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS