Native Chromatin Immunoprecipitation of Neurosphere Cells

Source: Mendez, F. M. et al., Native Chromatin Immunoprecipitation Using Murine Brain Tumor Neurospheres. J. Vis. Exp.  (2018).This video demonstrates the isolation of protein-DNA complexes from neurosphere cells derived from a mouse brain tumor using native chromatin immunoprecipitation. It details the steps for chromatin preparation, immunoprecipitation, washing, and protein digestion to release DNA for downstream analysis.

Publication Details

Journal
Journal of Visualized Experiments
Published
2025-07-24
DOI
https://doi.org/10.3791/31485
Primary Topic
RNA Research and Splicing
Type
article
Field-Weighted Citation Impact
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article

Native Chromatin Immunoprecipitation of Neurosphere Cells

Journal of Visualized Experiments
RNA Research and Splicing
article

Native Chromatin Immunoprecipitation of Neurosphere Cells

article en

Abstract

Source: Mendez, F. M. et al., Native Chromatin Immunoprecipitation Using Murine Brain Tumor Neurospheres. J. Vis. Exp.  (2018).This video demonstrates the isolation of protein-DNA complexes from neurosphere cells derived from a mouse brain tumor using native chromatin immunoprecipitation. It details the steps for chromatin preparation, immunoprecipitation, washing, and protein digestion to release DNA for downstream analysis.

Journal of Visualized Experiments
Zero hunger
Openalex Percentile: Top 83%
RNA Research and Splicing
DOISourceOpenAlex
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Native Chromatin Immunoprecipitation of Neurosphere Cells · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS