Optogenetic Stimulation and Electrophysiological Recording of Synaptic Events in Rat Brain Slices

Source: Kinnavane, L., et al. Ex Vivo Optogenetic Interrogation of Long-Range Synaptic Transmission and Plasticity from Medial Prefrontal Cortex to Lateral Entorhinal Cortex. J. Vis. Exp. (2022) This video demonstrates the procedure for studying synaptic efficiency in the lateral entorhinal cortex (LEC) by optogenetically stimulating medial prefrontal cortex (mPFC) axons and recording the responses of pyramidal neurons in the LEC. This method combines patch-clamp recordings with synaptic activation by light stimulation to study synaptic plasticity.

Publication Details

Journal
Journal of Visualized Experiments
Published
2025-07-24
DOI
https://doi.org/10.3791/31273
Primary Topic
Photoreceptor and optogenetics research
Type
article
Field-Weighted Citation Impact
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article

Optogenetic Stimulation and Electrophysiological Recording of Synaptic Events in Rat Brain Slices

Journal of Visualized Experiments
Photoreceptor and optogenetics research
article

Optogenetic Stimulation and Electrophysiological Recording of Synaptic Events in Rat Brain Slices

article en

Abstract

Source: Kinnavane, L., et al. Ex Vivo Optogenetic Interrogation of Long-Range Synaptic Transmission and Plasticity from Medial Prefrontal Cortex to Lateral Entorhinal Cortex. J. Vis. Exp. (2022) This video demonstrates the procedure for studying synaptic efficiency in the lateral entorhinal cortex (LEC) by optogenetically stimulating medial prefrontal cortex (mPFC) axons and recording the responses of pyramidal neurons in the LEC. This method combines patch-clamp recordings with synaptic activation by light stimulation to study synaptic plasticity.

Journal of Visualized Experiments
Zero hunger
Openalex Percentile: Top 80%
Photoreceptor and optogenetics research
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Optogenetic Stimulation and Electrophysiological Recording of Synaptic Events in Rat Brain Slices · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS