Optogenetic Electrophysiology Recording of Cells with Light-Dependent Ion Channels

Source: Grimm, C., et al. Whole-cell patch-clamp recordings for electrophysiological determination of ion selectivity in channelrhodopsins. J. Vis. Exp. (2017) In this video, embryonic kidney cell cultures expressing light-dependent ion channel proteins fused with a fluorescent reporter demonstrate the effect of light on ion movement. Whole-cell patch clamp is used to monitor the movement of ions.

Publication Details

Journal: Journal of Visualized Experiments
Published: 2025-07-24
DOI: https://doi.org/10.3791/31282
Primary Topic: Photoreceptor and optogenetics research
Type: article
Field-Weighted Citation Impact: 0.00

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article

Optogenetic Electrophysiology Recording of Cells with Light-Dependent Ion Channels

Jul 24, 2025

Journal of Visualized Experiments

Photoreceptor and optogenetics research

article

Optogenetic Electrophysiology Recording of Cells with Light-Dependent Ion Channels

July 24, 2025article en

Abstract

Journal of Visualized Experiments

Openalex Percentile: Top 80%

Photoreceptor and optogenetics research

DOI Source OpenAlex

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Optogenetic Electrophysiology Recording of Cells with Light-Dependent Ion Channels · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS