Time-Lapse Imaging to Monitor the Transcellular Spreading of Prion-Like Proteins in Transgenic Nematodes

Source: Nussbaum-Krammer, C. I., et al. Investigating the Spreading and Toxicity of Prion-like Proteins Using the Metazoan Model Organism C. elegans. J. Vis. Exp. (2015) This video demonstrates the procedure for in vivo imaging of C. elegans expressing prion-like proteins tagged with red fluorescent protein. The protocol involves immobilizing nematodes on an agarose pad for time-lapse confocal microscopy, revealing protein aggregation and transport within and between cells. This technique is crucial for studying protein aggregation related to neurodegenerative diseases.