Replating Differentiated Neurons Derived from Human Pluripotent Stem Cells

Source: Calabrese, B. et al., Post-differentiation Replating of Human Pluripotent Stem Cell-derived Neurons for High-content Screening of Neuritogenesis and Synapse Maturation. J. Vis. Exp. (2019) The video showcases a method to detach and replant human pluripotent stem cell-derived neurons using a mild proteolytic enzyme. After filtration and centrifugation, the neurons are replated on a coated multi-well plate for incubation, promoting attachment and maturation.