Characterization of Small Bowel Neuroendocrine Tumor Spheroids Using Immunofluorescence

Source: Ear, P. H., et. al. Establishment and Characterization of Small Bowel Neuroendocrine Tumor Spheroids. J. Vis. Exp. (2019)This video demonstrates the immunofluorescence staining of small bowel neuroendocrine tumor spheroids. The spheroids are fixed, permeabilized, and blocked and are then incubated with primary and secondary antibodies. A dye is added to stain nuclei before mounting and visualizing them under a fluorescent microscope.

Publication Details

Journal: Journal of Visualized Experiments
Published: 2025-07-24
DOI: https://doi.org/10.3791/31460
Primary Topic: Neuroendocrine Tumor Research Advances
Type: article
Field-Weighted Citation Impact: 0.00

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article

Characterization of Small Bowel Neuroendocrine Tumor Spheroids Using Immunofluorescence

Jul 24, 2025

Journal of Visualized Experiments

Neuroendocrine Tumor Research Advances

article

Characterization of Small Bowel Neuroendocrine Tumor Spheroids Using Immunofluorescence

July 24, 2025article en

Abstract

Journal of Visualized Experiments

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Neuroendocrine Tumor Research Advances

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Characterization of Small Bowel Neuroendocrine Tumor Spheroids Using Immunofluorescence · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS