Assessing the Internalization of Target Surface Proteins Using a Biotin Derivative

Source: Tham, D. K. L., et al., Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation. J. Vis. Exp. (2017). This video demonstrates a method to assess target protein internalization in the mouse cortical astrocytes using biotinylation, followed by cell lysis, streptavidin-based protein extraction, denaturation, and Western blot analysis to confirm successful internalization.