Isolation of Schwann Cells from Mouse Spinal Roots

Source: He, Q., et al., & Ding, F. Purification of fibroblasts and schwann cells from sensory and motor nerves in vitro. J. Vis. Exp. (2020) This video outlines the isolation and culturing of Schwann cells from mouse spinal roots. Isolated cells are then cultured in specific media. Schwann cells are isolated via differential digestion and adherence. They are subsequently transferred to extracellular matrix-coated coverslips for attachment and proliferation.