Assessing the Palmitoylation State of Cell Membrane Proteins from Mouse Brain Tissue

Source: Speca, D. J., et al. Acyl-PEGyl Exchange Gel Shift Assay for Quantitative Determination of Palmitoylation of Brain Membrane Proteins. J. Vis. Exp. (2020). The video demonstrates the analysis of the palmitoylation state of cell membrane proteins isolated from mouse brain tissue. The process involves exposing the non-palmitoylated cysteines and blocking them, then using a polymer that binds to the palmitoylated cysteines, aiding in assessing the palmitoylation state of the isolated proteins.

Publication Details

Journal: Journal of Visualized Experiments
Published: 2025-07-24
DOI: https://doi.org/10.3791/31440
Primary Topic: ATP Synthase and ATPases Research
Type: article
Field-Weighted Citation Impact: 0.00

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article

Assessing the Palmitoylation State of Cell Membrane Proteins from Mouse Brain Tissue

Jul 24, 2025

Journal of Visualized Experiments

ATP Synthase and ATPases Research

article

Assessing the Palmitoylation State of Cell Membrane Proteins from Mouse Brain Tissue

July 24, 2025article en

Abstract

Journal of Visualized Experiments

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Openalex Percentile: Top 83%

ATP Synthase and ATPases Research

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Assessing the Palmitoylation State of Cell Membrane Proteins from Mouse Brain Tissue · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS