Testing Monosynaptic Connections Using Tetrodotoxin and Tetrodotoxin-Resistant Sodium Channels

Source: Zhang, X. et al., Examining Monosynaptic Connections in Drosophila Using Tetrodotoxin Resistant Sodium Channels. J. Vis. Exp. (2018) This video demonstrates testing monosynaptic connections using a transgenic fly. The fly has tetrodotoxin-resistant sodium channels and red light-activated channels in its presynaptic neurons, which are optogenetically excited to transmit signals to postsynaptic neurons.