An In Vitro Model of Stretch Injury in Human Induced Pluripotent Stem Cell-Derived Neurons

Source: Phillips, J. K. et al. Method for High Speed Stretch Injury of Human Induced Pluripotent Stem Cell-derived Neurons in a 96-well Format. J. Vis. Exp. (2018) This video demonstrates the process of inducing stretch injury on human induced pluripotent stem cell-derived neurons (hiPSCNs). The cells are taken in a bottomless multi-well plate with a silicone membrane attached. Controlled mechanical stress is applied on the membrane to induce cytoskeletal disruption and cell membrane damage, inducing a stretch injury phenotype in the cells.