Analyzing Sleep Behavior in Mice through Electroencephalogram and Electromyogram Recordings

Source: Oishi, Y., et  al. Polygraphic Recording Procedure for Measuring Sleep in Mice. J. Vis. Exp. (2016). This video demonstrates a protocol for implanting an electroencephalogram (EEG) and electromyogram (EMG) electrode assembly in an anesthetized mouse. The process involves securing the mouse on a stereotaxic frame, making a midline incision in the skull, drilling holes for the electrodes, and fixing the electrode unit for signal recording. After recovery, the mouse's sleep-wake behavior is analyzed using the recorded signals.