Automated Image-Guided Patch Clamp Technique for Studying Neurons in Brain Slices

Source: Wu, Q., et al. Application of automated image-guided patch clamp for the study of neurons in brain slices. J. Vis. Exp. (2017). In this video, brain slices are immobilized in a recording chamber, and cells of interest are located and focused on using a computer-controlled microscope. The automated image processing system then precisely positions a patch pipette to seal the cell membrane and monitor electrical signals.